PA53-FL & Image-Pro® Analysis Package
AI Package
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This Research Package includes:
- PA53 BIO FS6 w/ LUMOS FL LED
- Moticam ProS5 Plus USB Camera
- Image-Pro ® - Life Science Fluorescence Package for Motic (2D)
- 0.65X C-Mount camera adapter
*Advanced AI software is available for this package
- Descriptions
- Microscope Specifications
- Camera Specifications
- Image-Pro Software Features
- Downloads
- Reviews
The PA53 BIO FS6 models offer maximum flexibility for managing any kind of Fluorescence application thanks to the LUMOS High-power LED FL light source, with individual control of each LED channel. The Fluorescence filter cubes are mounted in an encoded 6-position turret: multi-color staining can easily be handled in combination with our channel-merge software plug-in. The standard configuration of PA53 BIO FS6 comes with DAPI, FITC, and TRITC filter cubes but many more can be added. Our CCIS® HPlan S-APO (High Fluorite Class) objectives provide high transmission rates for the brightest images.
The Hi-Sensitives - Moticam ProS5 Plus is characterized by a large 2/3' sCMOS sensor and a Global Shutter for speedy handling of moving phenomena. This camera line takes Sensitivity as a priority: Fluorescence, Polarization, and Darkfield are the applications the engineers had in mind.
Unlock the full potential of your fluorescence microscope with the Image-Pro ® - Life Science Fluorescence Package for Motic, developed in partnership with Media Cybernetics. This all-in-one solution allows users to capture mixed sets of fluorescence and brightfield channels, then deconvolve them using AutoQuant Real-Time Deconvolution. Combining advanced Machine Learning technology with a comprehensive suite of protocols provides precise segmentation, classification, and automated measurements from 2D fluorescence images.
This package also includes a 12-month subscription to a Standard Success Plan from Media Cybernetics, providing ongoing technical assistance and software onboarding so you can focus on the work that truly matters.
| Model | PA53 BIO FS6 |
| Optical System | Colour Corrected Infinity Optical System (CCIS®) |
| Observation tube | Trinocular head, Siedentopf type |
| Inclination | 30° inclined |
| Trinocular light split | 100:0/20:80/0:100 |
| Interpupillary distance | 50-75mm |
| Diopter adjustment | On both eyepieces, +/- 4 diopter |
| Eyepieces | Widefield WF10X/23mm with diopter adjustment |
| Intermediate body | Epi-Fluorescence illuminator LUMOS LED with 6 position coded fluorescence turret and DAPI, FITC and TRITC filter cubes mounted |
| Nosepiece | Reversed quintuple, coded |
| Objective classification | CCIS® HPlan S-APO (Fluor) |
| Objectives | • 4X/0.13 (WD 17.3mm) • 10X/0.3 (WD 11.7mm) • 20X/0.5 (WD 2.2mm) • 40X/0.75/S (WD 0.7mm) • 100X/1.3/S-Oil (WD 0.1mm) |
| Objective mounting thread | W 4/5"x1/36" (RMS standard) |
| Stand type | Upright |
| Stage | Mechanical stage with built-in low position rackless coaxial stage control and sample holder |
| Stage size | 220x170mm |
| Travel range X&Y | 80x55mm |
| Condenser | Focusable and centerable Abbe condenser N.A. 0.90/1.25 with slot for contrast sliders |
| Diaphragm | Iris diaphragm |
| Focus mechanism | Coaxial coarse and fine focusing system with tension adjustment |
| Fine focus precision | 1µm |
| Focusing stroke | 29.5mm - Coarse: 17.7mm/revolution - Fine: 0.1mm/revolution (1µm scale) |
| Upper limit stop | Upper limit stop preset but adjustable |
| Filter | ND6, ND25, LBD |
| Filter holder | Integrated filters |
| Incident illumination | LUMOS High-power LED FL light source (Optional: Mercury light source) |
| Transmitted illumination | Köhler Quartz halogen 12V/100W with intensity control |
| Illumination features | Power saving mode ECO function, LED voltage indicator and Intelligent Light function |
| Software | Motic Images Plus 3.0 software with plug-in FL channel merge |
| Transformer | Internal |
| Power supply | 110-240V (CE) |
| Accessories included | Dust cover, power cord, Allen key, immersion oil (5ml), 2 empty filter cubes, UV light blocking tube, UV light protective shield |
| Dimensions LxWxH | 648x247x513mm |
| Net weight | 24.2kg |
| Contrast techniques | |
| Standard contrast technique | Brightfield |
| Phase contrast | Optional slider/turret |
| Polarization | Optional add-on |
| Darkfield | Optional slider/turret |
| Fluorescence | LED |
| Model | Moticam ProS5 Plus |
| Sensor type | sCMOS |
| Sensor size | 2/3" |
| Imaging area | 11.1mm (Diagonal) |
| Capture resolution info | 5MP |
| Live display mode through USB (pixels) | 2448x2048, 1224x1024 |
| Pixel size | 3.45x3.45μm |
| Scan mode | Progressive |
| Shutter mode | Global Shutter |
| Data transfer | USB 3.1 |
| Max. frames per second (fps*) | 2448x2048 @ 68.3fps, 1224x1024 @ 175.8fps |
| Exposure time | 7μsec to 2 sec |
| Operating temperature | From -10 to +60 Degrees Celsius non condensing |
| Sensitivity | 1146mV(G) @ 1/30 sec |
| Support device | TWAIN, SDK and DirectShow Driver |
| Supported OS | Microsoft Windows 7/8/10, MAC OSX, Linux or higher |
| Minimum computer requirements | 2GHz dualcore - RAM memory 2GB - Video memory min. 512 MB |
| Lens mount | C-Mount |
| Software | Motic Images Plus 3.0 for Windows, OSX and Linux |
| Functions | Still image and video capture, live and still image measurement, image adjustments, white balance, automatic and manual exposure, individual objective calibration system |
| Power supply | 5V (from USB Port) |
| Package includes | CS ring adaptor, USB 3.1 cable, Motic 4-dot calibration slide, Motic Images Plus 3.0 for Windows/OSX/Linux |
| (*) Frames per second under optimal lighting conditions and in compliance with computer technical requirements. | |
 |
Image-Pro® Software Package by Media Cybernetics
|
| Software Key Features | Standard Package | AI-Powered Package |
|
AI Deep Learning-Powered Analysis For superior 2D image segmentation accuracy over Machine Learning and other methods, this library of Pre-Trained AI models delivers instant segmentation results for a wide range of applications.
|
✓ | |
|
AI Deep Learning Model Training Fine tune the existing Pre-Trained models or build your own for custom segmentation of the most challenging images.
|
✓ | |
|
Machine Learning-Powered Analysis Leverage sophisticated pixel classification algorithms for accurate segmentation and classification, ensuring automated and reliable measurements from 2D images.
|
✓ | ✓ |
|
Analysis Protocols for guided analysis Streamline your research with a comprehensive suite of protocols designed for efficient, repeatable analysis across various life science applications—all within a simple, step-by-step workflow.
Includes: Angiogenesis, Cell Apoptosis, Autophagy, Confluence, Cell Count, Cell Morphology, Cell Proliferation, Transfection, Colocalization, Lipid Droplets, Live/Dead, Neurite Outgrowth, Nuclei, Ring Regions, Translocation, and Wound Healing. |
✓ | ✓ |
|
Data Visualization and Reporting Choose from 144+ measurements to display in tables and graphs, generate custom reports, and export data to Excel, CSV, or PDF. Easily share individual measurements and statistics with collaborators.
|
✓ | ✓ |
|
Image Processing Correct background illumination, tile and stitch large areas, colorize your images, enhance with filters, align and register images, and more. Choose from a helpful suite of options to prepare your images for analysis or publication.
|
✓ | ✓ |
|
Multi-Channel Fluorescence Capture Control the LUMOS LED light source with easy guided prompts to capture multiple fluorescence channels into a single multi-channel image, with Auto Exposure, and Real-Time Deconvolution.
|
✓ | ✓ |
|
Image Preview and Capture Designed to control the Moticam camera, you can now automatically preview a live image and effortlessly capture LIVE Tiling, LIVE EDF, LIVE HDR, Timelapse movies, and beautiful still images.
|
✓ | ✓ |
Check compatibility with Image-Pro
| Different packages have varying system requirements. Check your computer’s specifications against the system requirements for Image-Pro software. | See System Requirements | |
Microscope: PA53 BIO FS6
Camera: Moticam ProS5 Plus
Descriptions
The PA53 BIO FS6 models offer maximum flexibility for managing any kind of Fluorescence application thanks to the LUMOS High-power LED FL light source, with individual control of each LED channel. The Fluorescence filter cubes are mounted in an encoded 6-position turret: multi-color staining can easily be handled in combination with our channel-merge software plug-in. The standard configuration of PA53 BIO FS6 comes with DAPI, FITC, and TRITC filter cubes but many more can be added. Our CCIS® HPlan S-APO (High Fluorite Class) objectives provide high transmission rates for the brightest images.
The Hi-Sensitives - Moticam ProS5 Plus is characterized by a large 2/3' sCMOS sensor and a Global Shutter for speedy handling of moving phenomena. This camera line takes Sensitivity as a priority: Fluorescence, Polarization, and Darkfield are the applications the engineers had in mind.
Unlock the full potential of your fluorescence microscope with the Image-Pro ® - Life Science Fluorescence Package for Motic, developed in partnership with Media Cybernetics. This all-in-one solution allows users to capture mixed sets of fluorescence and brightfield channels, then deconvolve them using AutoQuant Real-Time Deconvolution. Combining advanced Machine Learning technology with a comprehensive suite of protocols provides precise segmentation, classification, and automated measurements from 2D fluorescence images.
This package also includes a 12-month subscription to a Standard Success Plan from Media Cybernetics, providing ongoing technical assistance and software onboarding so you can focus on the work that truly matters.
Microscope Specifications
| Model | PA53 BIO FS6 |
| Optical System | Colour Corrected Infinity Optical System (CCIS®) |
| Observation tube | Trinocular head, Siedentopf type |
| Inclination | 30° inclined |
| Trinocular light split | 100:0/20:80/0:100 |
| Interpupillary distance | 50-75mm |
| Diopter adjustment | On both eyepieces, +/- 4 diopter |
| Eyepieces | Widefield WF10X/23mm with diopter adjustment |
| Intermediate body | Epi-Fluorescence illuminator LUMOS LED with 6 position coded fluorescence turret and DAPI, FITC and TRITC filter cubes mounted |
| Nosepiece | Reversed quintuple, coded |
| Objective classification | CCIS® HPlan S-APO (Fluor) |
| Objectives | • 4X/0.13 (WD 17.3mm) • 10X/0.3 (WD 11.7mm) • 20X/0.5 (WD 2.2mm) • 40X/0.75/S (WD 0.7mm) • 100X/1.3/S-Oil (WD 0.1mm) |
| Objective mounting thread | W 4/5"x1/36" (RMS standard) |
| Stand type | Upright |
| Stage | Mechanical stage with built-in low position rackless coaxial stage control and sample holder |
| Stage size | 220x170mm |
| Travel range X&Y | 80x55mm |
| Condenser | Focusable and centerable Abbe condenser N.A. 0.90/1.25 with slot for contrast sliders |
| Diaphragm | Iris diaphragm |
| Focus mechanism | Coaxial coarse and fine focusing system with tension adjustment |
| Fine focus precision | 1µm |
| Focusing stroke | 29.5mm - Coarse: 17.7mm/revolution - Fine: 0.1mm/revolution (1µm scale) |
| Upper limit stop | Upper limit stop preset but adjustable |
| Filter | ND6, ND25, LBD |
| Filter holder | Integrated filters |
| Incident illumination | LUMOS High-power LED FL light source (Optional: Mercury light source) |
| Transmitted illumination | Köhler Quartz halogen 12V/100W with intensity control |
| Illumination features | Power saving mode ECO function, LED voltage indicator and Intelligent Light function |
| Software | Motic Images Plus 3.0 software with plug-in FL channel merge |
| Transformer | Internal |
| Power supply | 110-240V (CE) |
| Accessories included | Dust cover, power cord, Allen key, immersion oil (5ml), 2 empty filter cubes, UV light blocking tube, UV light protective shield |
| Dimensions LxWxH | 648x247x513mm |
| Net weight | 24.2kg |
| Contrast techniques | |
| Standard contrast technique | Brightfield |
| Phase contrast | Optional slider/turret |
| Polarization | Optional add-on |
| Darkfield | Optional slider/turret |
| Fluorescence | LED |
Camera Specifications
| Model | Moticam ProS5 Plus |
| Sensor type | sCMOS |
| Sensor size | 2/3" |
| Imaging area | 11.1mm (Diagonal) |
| Capture resolution info | 5MP |
| Live display mode through USB (pixels) | 2448x2048, 1224x1024 |
| Pixel size | 3.45x3.45μm |
| Scan mode | Progressive |
| Shutter mode | Global Shutter |
| Data transfer | USB 3.1 |
| Max. frames per second (fps*) | 2448x2048 @ 68.3fps, 1224x1024 @ 175.8fps |
| Exposure time | 7μsec to 2 sec |
| Operating temperature | From -10 to +60 Degrees Celsius non condensing |
| Sensitivity | 1146mV(G) @ 1/30 sec |
| Support device | TWAIN, SDK and DirectShow Driver |
| Supported OS | Microsoft Windows 7/8/10, MAC OSX, Linux or higher |
| Minimum computer requirements | 2GHz dualcore - RAM memory 2GB - Video memory min. 512 MB |
| Lens mount | C-Mount |
| Software | Motic Images Plus 3.0 for Windows, OSX and Linux |
| Functions | Still image and video capture, live and still image measurement, image adjustments, white balance, automatic and manual exposure, individual objective calibration system |
| Power supply | 5V (from USB Port) |
| Package includes | CS ring adaptor, USB 3.1 cable, Motic 4-dot calibration slide, Motic Images Plus 3.0 for Windows/OSX/Linux |
| (*) Frames per second under optimal lighting conditions and in compliance with computer technical requirements. | |
Image-Pro Software Features
|
|
Image-Pro® Software Package by Media Cybernetics
|
| Software Key Features | Standard Package | AI-Powered Package |
|
AI Deep Learning-Powered Analysis For superior 2D image segmentation accuracy over Machine Learning and other methods, this library of Pre-Trained AI models delivers instant segmentation results for a wide range of applications.
|
✓ | |
|
AI Deep Learning Model Training Fine tune the existing Pre-Trained models or build your own for custom segmentation of the most challenging images.
|
✓ | |
|
Machine Learning-Powered Analysis Leverage sophisticated pixel classification algorithms for accurate segmentation and classification, ensuring automated and reliable measurements from 2D images.
|
✓ | ✓ |
|
Analysis Protocols for guided analysis Streamline your research with a comprehensive suite of protocols designed for efficient, repeatable analysis across various life science applications—all within a simple, step-by-step workflow.
Includes: Angiogenesis, Cell Apoptosis, Autophagy, Confluence, Cell Count, Cell Morphology, Cell Proliferation, Transfection, Colocalization, Lipid Droplets, Live/Dead, Neurite Outgrowth, Nuclei, Ring Regions, Translocation, and Wound Healing. |
✓ | ✓ |
|
Data Visualization and Reporting Choose from 144+ measurements to display in tables and graphs, generate custom reports, and export data to Excel, CSV, or PDF. Easily share individual measurements and statistics with collaborators.
|
✓ | ✓ |
|
Image Processing Correct background illumination, tile and stitch large areas, colorize your images, enhance with filters, align and register images, and more. Choose from a helpful suite of options to prepare your images for analysis or publication.
|
✓ | ✓ |
|
Multi-Channel Fluorescence Capture Control the LUMOS LED light source with easy guided prompts to capture multiple fluorescence channels into a single multi-channel image, with Auto Exposure, and Real-Time Deconvolution.
|
✓ | ✓ |
|
Image Preview and Capture Designed to control the Moticam camera, you can now automatically preview a live image and effortlessly capture LIVE Tiling, LIVE EDF, LIVE HDR, Timelapse movies, and beautiful still images.
|
✓ | ✓ |
Check compatibility with Image-Pro
| Different packages have varying system requirements. Check your computer’s specifications against the system requirements for Image-Pro software. | See System Requirements | |
Downloads
Microscope: PA53 BIO FS6
Camera: Moticam ProS5 Plus